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KMID : 0665220180310060836
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Korean Journal of Food and Nutrition
2018 Volume.31 No. 6 p.836 ~ p.843
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Influence of High Hydrostatic Pressure Treatment after Germination on Anti-proliferation Effects of Soyasaponin-rich Fraction in Black Soybean (Glycine max L.)
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Kim Min-Young
Lee Yoon-Jeong
Song Myeong-Seob
Oh Hyun-Ah
Kim Kyung-Mi
Kang Tae-Su
Lee Youn-Ri
Lee Jun-Soo
Jeong Heon-Sang
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Abstract
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The objective of this study was to determine the effect of high hydrostatic pressure (HHP) treatment on proliferation of human cancer cell lines (MCF-7, HCT-116, PC-3 and AGS) of crude soyasaponin extracts in germinated black soybean.
Black soybean was germinated and subjected to HHP, followed by preparation of crude soyasaponin extracts. Cell treatments done with extracts less than 400 μg/mL concentrations had no significant effect on 3T3-L1 adipocyte cell viability. The inhibitory effect of crude soyasaponin extracts with germination periods and applied pressure on breast cancer cell (MCF-7), human colon cancer cell (HCT-116), human gastriccancer cell (AGS) and prostate cancer cell (PC-3) growth were investigated using MTT assay. The highest anti-proliferation of human cancer cell line of crude soyasaponin extracts was observed at 150 MPa treatment after germination for 4 days (150 MPa-Day 4). The cell viability on MCF-7, HCT-116, PC-3 and AGS cell lines of crude soyasaponin extract in 150 MPa-Day4 was 48.82%, 57.37%, 39.89% and 23.94% at 400 μg/mL, respectively. These results suggest that soyasaponin extracts from black soybean subjected to HHP after germination may mediate physiological activity.
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KEYWORD
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black soybean, soyasaponin, germination, high hydrostatic pressure treatment, anticancer activity
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